eMethods 1. Sample Size and Power Calculations
eMethods 2. Ethical Approval, Inclusion and Exclusion Criteria, and Data Collection Procedures
eMethods 3. Description of Measurement of Study Variables
eFigure. Flow of Participants From Enrollment to the Analytic Cohort
eTable 1. Comparison of Two Vaginal and Two Cesarean Modes of Delivery on Main Outcomes (Conception and Live Birth)
eTable 2. Comparison of Women Who Were Lost to Follow-Up to Women Who Were Retained to the 36-Month Survey
eTable 3. Association of Baseline Characteristics and Loss to Follow-Up, Multivariable Analysis
eTable 4. Pre-exposure Covariates by Mode of First Delivery and Subsequent Conception Among Women Who Had Unprotected Intercourse Before First Conception or Resulting in No Conception
eTable 5. Concurrent-exposure Covariates by Mode of First Delivery and Subsequent Conception Among Women Who Had Unprotected Intercourse Before First Conception or Resulting in No Conception
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Kjerulff KH, Paul IM, Weisman CS, et al. Association Between Mode of First Delivery and Subsequent Fecundity and Fertility. JAMA Netw Open. 2020;3(4):e203076. doi:10.1001/jamanetworkopen.2020.3076
¿Existe relación entre un primer parto por cesárea y una tasa más baja de concepción posterior en comparación con el parto vaginal?
En este estudio de cohorte de 2423 mujeres, el primer parto por cesárea se relacionó con una tasa más baja de concepción después de tener relaciones sexuales sin protección en comparación con las tasas de concepción de las mujeres después del parto vaginal. Estos resultados se mantuvieron significativos después de controlar las covariables relevantes.
Estos hallazgos sugieren que las mujeres que dan a luz a su primer hijo por cesárea pueden tener menos probabilidades de concebir un segundo hijo en los 3 años posteriores al primer parto que las mujeres que dan a luz a su primer hijo por vía vaginal.
More than 20% of births globally are by cesarean delivery, including more than 30% in the US. Prior studies have reported lower rates of childbearing after cesarean delivery, but it is not clear if this is due to maternal choice or lower conception rates.
To investigate the association between mode of first delivery and subsequent conceptions and live births.
Design, Setting, and Participants
The First Baby Study was a multicenter prospective cohort study of women aged 18 to 35 years with singleton pregnancies, enrolled and interviewed before first childbirth, who delivered in Pennsylvania from 2009 to 2011 and were followed up for 36 months after delivery (until April 2014). Data analysis for this study took place between May and July 2019 and in January 2020.
Mode of first delivery (cesarean or vaginal).
Main Outcomes and Measures
Rates of subsequent conceptions and live births. Discrete-time Cox proportional hazard regression models were used to compare the rate of subsequent conception (vaginal vs cesarean) among those who completed the 36-month survey, accounting for reported months of unprotected intercourse during the follow-up period and adjusting for relevant covariates. A log binomial regression was used to compare the age-adjusted rate of subsequent live birth (vaginal vs cesarean) among those who completed the 36-month survey.
The study population consisted of 2423 women who were retained to the 36-month survey (mean [SD] age at baseline was 27.2 [4.4] years and 712 [29.4%] delivered by cesarean). There were 2046 women who had unprotected intercourse during the follow-up period, 2021 of whom provided data on months of unprotected intercourse. Cesarean delivery was associated with lower rates of conception after unprotected intercourse during the follow-up period (413 of 599 [68.9%]) compared with vaginal delivery (1090 of 1422 [76.7%]) (adjusted hazard ratio, 0.85; 95% CI, 0.74-0.96). Cesarean delivery was also associated with reduced likelihood of a subsequent live birth (305 women [42.8%]) compared with vaginal delivery (857 women [50.1%]), with an age-adjusted risk ratio of 0.83 (95% CI, 0.75-0.92).
Conclusions and Relevance
In the 3 years following first childbirth, women who delivered their first child by cesarean had lower rates of conception after unprotected intercourse, and fewer of these women had a second child than those who delivered vaginally.
The global average annual cesarean delivery rate has more than tripled over the past 25 years, from 6.7% in 1990 to 21.1% in 2015.1,2 In the US, nearly a third of deliveries are by cesarean (31.9% in 2018), with rates above 36.0% in several states.3 Most studies examining the association between mode of delivery and subsequent childbearing conducted over the past 35 years have reported lower rates of childbearing after cesarean delivery compared with vaginal delivery.4-6 A meta-analysis6 that included nearly 600 000 women reported that women who had cesarean delivery were 9% less likely to have a subsequent pregnancy (age-adjusted risk ratio [RR], 0.91; 95% CI, 0.87-0.95) and 11% less likely to have a subsequent live birth (age-adjusted RR, 0.89; 95% CI, 0.87-0.92). Some have hypothesized that women who deliver by cesarean are less likely to want additional children or more likely to decide to delay subsequent childbearing,7-11 while others have hypothesized that women who deliver by cesarean are less likely to conceive subsequently,12-15 either because of the factors that predispose them to need cesarean delivery or because of the cesarean delivery itself.12-14 Because most of the previous studies of childbearing subsequent to cesarean delivery were retrospective analyses of existing data sets designed for other purposes, such as birth certificate data,8,16-20 or were surveys conducted years after the index delivery,7,21,22 it is not clear if women who deliver by cesarean are less likely to plan to have additional children and subsequently do not try to conceive, or are simply less likely to conceive.
We designed a large-scale prospective cohort interview study to investigate factors associated with subsequent childbearing following first childbirth. The goals of this study were to compare women who delivered their first child vaginally with those who delivered by cesarean in terms of fecundity (“the biologic capacity for reproduction, irrespective of pregnancy intention”23) and fertility (“demonstrated fecundity, measured by live births”23).
The First Baby Study was a prospective, multicenter, cohort study of nulliparous women who were enrolled during pregnancy and followed up through 36 months post partum. The participants were interviewed during pregnancy (baseline) and then at 1, 6, 12, 18, 24, 30, and 36 months post partum. Key objectives of the postpartum interviews were to measure unprotected intercourse in each of the 36 months of follow-up and resulting conceptions, including those that did not result in a live birth. Women who dropped out of the study before the 36-month survey were missing data concerning unprotected intercourse, as well as conceptions and pregnancy outcomes, for at least 6 of the 36 months of follow-up (and generally more). Therefore, this study was designed and powered to include only those women who were retained to the 36-month data collection stage. Details about sample size and power calculations, participant recruitment, and data collection procedures have been published elsewhere24 and are also provided in eMethods 1 and eMethods 2 in the Supplement.
This study was approved by the institutional review board of the Penn State University College of Medicine, as well as the review boards of all hospitals and other institutions involved with participant recruitment. All participants provided written informed consent. The study followed the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) reporting guidelines.
There were 2 main outcomes measured during the 36 months of follow-up: (1) conception (whether participants conceived after unprotected intercourse) among the subset of women who were retained to the 36-month data collection stage and reported having unprotected intercourse during the follow-up period, for the purpose of comparing women who delivered their first child vaginally with those who delivered by cesarean, and (2) 1 or more live births among all of the women who were retained to the 36-month data collection stage, to compare the women who delivered their first child vaginally with those who delivered by cesarean. The secondary outcomes included number of pregnancies, live births, miscarriages, abortions and stillbirths, months of unprotected intercourse before first conception or resulting in no conception, becoming pregnant for the first time since first delivery at the time of the 36-month survey, and whether women sought fertility advice, testing, or treatment during the follow-up period. There were a small number of women who, for various reasons (such as being in same-sex relationships), used artificial insemination or other types of fertility treatment to attempt conception of 1 or more subsequent children. For these women, we classified the number of months of attempted conception via fertility treatment as number of months of unprotected intercourse.
Preexposure covariates (factors preceding delivery) investigated included demographic and psychosocial factors (stress, depression, and social support), plans to have another child within 3 years, health history (including prepregnancy body mass index, time to conception of first child, and gestational weight gain more than recommended25), pregnancy complications, and indications for cesarean delivery. Concurrent exposure covariates included labor and delivery–related complications, as well as in-hospital maternal and newborn morbidities. Additional details describing the assessment of all outcomes and covariates are provided in eMethods 3 in the Supplement.
Statistical analyses were performed using SPSS version 24.0 (IBM/SPSS Inc) and SAS version 9.4 (SAS Institute Inc). The statistical analyses for this article were performed between May and July 2019 and in January 2020. This study was designed and powered to compare childbearing subsequent to 2 modes of delivery, vaginal vs cesarean, as have most previous studies of mode of delivery in relation to subsequent childbearing.4-6 However, we compared spontaneous with instrumental vaginal delivery and planned cesarean with unplanned cesarean for our 2 study outcomes to determine whether there were mode of delivery subgroup differences that we would need to adjust for. The 2 vaginal delivery modes (spontaneous and instrumental) were not significantly different at the study’s threshold for statistical significance (2-tailed P < .05) on the study outcomes, nor were the 2 cesarean delivery modes (planned and unplanned) (eTable 1 in the Supplement).
We adjusted for clustering within delivery hospitals.26,27 For the outcome of rate of conception after unprotected intercourse, we used discrete-time Cox proportional hazards models28 to estimate the hazard ratios (HRs) and 95% CIs among women who had unprotected intercourse during the follow-up period and reported months of unprotected intercourse. We measured the associations between the potential covariates and the exposure variable (mode of delivery), as well as the associations between the potential covariates and the primary outcome variable (conception after unprotected intercourse) via χ2 analyses to determine which variables to include in the HR models as confounders. Variables that were associated (at P ≤ .10) with both the exposure variable and the outcome variable were included as covariates in the Cox proportional hazard models. The association between mode of first delivery and subsequent conception was modeled in stages as follows: model 1 adjusted for age, model 2 adjusted for age and preexposure covariates, and model 3 adjusted for these factors as well as concurrent-exposure covariates. The time variable in these analyses was months of unprotected intercourse. Because the primary objective of this study was to measure fecundity, women were included in these analyses if they reported having unprotected intercourse and reported the number of months of unprotected intercourse during the follow-up period, regardless of whether they reported that they were trying to conceive or not. We constructed curves showing the crude unadjusted cumulative percentage of women who conceived during the follow-up period in association with the number of months of unprotected intercourse for the women who delivered their first child vaginally and by cesarean.
Log binomial regression models were used to estimate the unadjusted and age-adjusted RRs and 95% CIs of a second live birth by 36 months post partum, comparing vaginal with cesarean delivery among all women who completed the 36-month survey. There were no missing data for the main outcomes of conception and subsequent live births within 36 months among the women who completed the 36-month survey, and little or no missing data for the study covariates (<1%). We compared those who were lost to follow-up with those who were retained in terms of maternal age, race/ethnicity, education, poverty status, marital status, mode of delivery, pregnancy intendedness of the first child, and plans to have another child within 3 years after the birth of the first child, via χ2 analyses and logistic regression.
From the enrolled sample of 3006 women, 2423 women (80.1%) completed the 36-month follow-up (eFigure in the Supplement), of whom 2046 (84.4%) reported having unprotected intercourse during the follow-up period, and 2021 (83.4%) women provided complete data on months of unprotected intercourse. There was no significant difference in attrition by delivery mode (eTable 2 in the Supplement). The factors most strongly associated with loss to follow-up, based on logistic regression, were nonwhite race, having less than a college degree, and being unmarried (eTable 3 in the Supplement).
The characteristics of the 2423 study participants can be seen in Table 1. There were 712 women who delivered their first child by cesarean (29.4%), and the mean (SD) age at baseline was 27.2 (4.4) years. Those who delivered by cesarean were older, more likely to be overweight and obese, shorter, and more likely to have sought fertility advice, testing, or treatment. Women did not differ by mode of delivery in prenatal plans for subsequent childbearing. As shown in Table 2, women did not differ by mode of delivery in whether they planned to have a subsequent child during the coming 3 years as reported at 1 month post partum (1009 women [59%] in the vaginal group vs 408 women [57.3%] in the cesarean group; P = .47); whether or not they had unprotected intercourse during the follow-up period (1443 women [84.3%] in the vaginal group vs 603 women [84.7%] of the cesarean group; P = .85); the mean (SD) age of the first child when the women first began having unprotected intercourse (13.5 [10.2] months for the vaginal group vs 13.2 [10.1] months for the cesarean group; P = .64); or the mean (SD) frequency of unprotected intercourse per month in the months when they were sexually active (5.1 [3.4] times per month for the vaginal group vs 4.8 [3.3] times per month for the cesarean group; P = .12).
Compared with vaginal births, women whose first delivery was by cesarean were less likely to conceive after unprotected intercourse, whether or not they were trying to conceive (Table 3). Among the 2423 women in the study, 305 (42.8%) whose first delivery was by cesarean had 1 or more live births during the 36-month follow-up period, compared with the 857 women (50.1%) whose first delivery was vaginal. The unadjusted RR was 0.85 (95% CI, 0.77-0.94) and the age-adjusted RR was 0.83 (95% CI, 0.75-0.92). Mode of first delivery was not associated with subsequent miscarriage or abortion, but women who had delivered by cesarean had a higher subsequent stillbirth rate than those who had delivered vaginally (6 of 509 [1.2%] vs 2 of 1393 [0.1%]; P = .01).
As can be seen in eTables 4 and 5 in the Supplement, the preexposure and concurrent-exposure covariates that were significantly associated with both mode of first delivery and subsequent conception among women who had unprotected intercourse were maternal age, prepregnancy body mass index, time to conception of the first child, gestational weight gain (more than recommended by Institute of Medicine guidelines25), prior induced abortions, diabetes, hypertension, soft-tissue disorders, hospitalization during pregnancy, dystocia, failed induction, and an Apgar score below 9/10 at 5 minutes.
Among the 2043 women who reported having unprotected intercourse, 25 reported that they had unprotected intercourse but did not report the months of unprotected intercourse, leaving 2021 women for the analyses involving months of unprotected intercourse, including the discrete-time HR models. Among these women, 599 (29.6%) had delivered their first child by cesarean. Among those who had delivered vaginally, 1090 (76.7%) conceived after unprotected intercourse, while 413 (68.9%) of those who had delivered by cesarean conceived after unprotected intercourse (P < .001). As shown in the Figure, women who delivered by cesarean were less likely to conceive than women who delivered vaginally at each value of number of months of unprotected intercourse. Cesarean delivery was associated with a significantly lower rate of conception after unprotected intercourse (age-adjusted HR, 0.77; 95% CI, 0.69-0.87), as shown in Table 4. This association remained significant after adjustment for all covariates (HR, 0.85; 95% CI, 0.74-0.96). In the final model, the following variables were associated with lower rates of conception: cesarean delivery, higher prepregnancy body mass index, taking more than a year to conceive the first child, failed induction at first childbirth, and prior abortions.
In this large-scale prospective cohort study of women interviewed before first childbirth and followed for 36 months post partum, we found that cesarean delivery was associated with lower rates of conception after unprotected intercourse during the 36-month follow-up period, and with less likelihood of having a subsequent child than women who had delivered vaginally, even though women who delivered by cesarean were just as likely to plan to have a subsequent child within 3 years after first childbirth, to have unprotected intercourse in the 36 months following first delivery, and to begin having unprotected intercourse at an average of 13 months after first delivery. Although we found that first delivery by cesarean was associated with higher likelihood of subsequent stillbirth compared with vaginal delivery, the number of stillbirths was too small to account for the mode of delivery difference in rate of subsequent live births. These results are in agreement with several previous studies that reported higher stillbirth rates after cesarean delivery.30-32
While prepregnancy body mass index and time to conception before first childbirth were significant confounding variables in the multivariable models, even after we controlled for these and the other covariates, mode of delivery remained significantly associated with fecundity. In addition, we found this mode of delivery difference in subsequent conception rates even among the women who were having unprotected intercourse while not trying to conceive. However, it is likely that there are other factors involved with fecundity following cesarean delivery that we did not study. In recent years it has been reported that some women develop a defect at the site of the cesarean incision (referred to as isthmocele or niche) that may increase risk of subsequent infertility.33 Such cesarean scar defects have been observed in 61% of women after a single cesarean delivery, based on transvaginal ultrasonographic examination.34 It has been reported that the implantation rate and pregnancy rate after in vitro fertilization are significantly lower among women with prior cesarean delivery than those with prior vaginal delivery.35
The results of this study are consistent with previous studies, which have reported lower rates of childbearing after cesarean compared with vaginal delivery.5,6,18 However, there was 1 previous study of a cohort of primiparous Danish women who were trying to conceive that reported no difference in rate of conception when comparing women who had delivered by unplanned cesarean (112 women) with those who had spontaneous vaginal delivery (585 women), although in that study women who had attempted pregnancy for more than 6 menstrual cycles were excluded.36
To our knowledge, this is the first prospective study beginning before first childbirth that measured unprotected intercourse in each month over the course of 36 months after first childbirth, to test whether women who delivered by cesarean were less likely to try to conceive subsequently than women who delivered vaginally, as has been hypothesized, or less likely to conceive after unprotected intercourse. The prospective design of this study was an important strength because we were able to measure women’s plans for subsequent childbearing before and after first childbirth, as well as unprotected intercourse in each month subsequent to first delivery among women who were trying to conceive and those who were not. Additional strengths of this study included a large sample size with adequate power to reject the null hypothesis, and a high retention rate.
This study has several limitations. First, the participants in this study were from 1 state in 1 country, which limits the generalizability of the findings. Second, the 3006 women who were enrolled were of higher socioeconomic status than in the general population of women at first childbirth in the state,24 and the 2423 women who were retained to the final survey were of higher socioeconomic status than the 583 women who were lost to follow-up. Bias caused by self-selection and attrition limit the internal validity of an etiologic study if associated with both exposure and outcome.37 However, neither self-selection into the study nor attrition was associated with the exposure variable, mode of delivery. Third, the study outcomes of birth control use in each month, unprotected intercourse, pregnancy, stillbirth, miscarriage, and abortion were self-reported, and therefore subject to recall bias and imprecision in the assessment of these factors. Fourth, we only followed women for a period of 3 years after first childbirth. Therefore, we cannot speak to the likelihood that the women in our study who delivered by cesarean would eventually catch up to the women who delivered vaginally in terms of fecundity and fertility. However, long-term studies of mode of first delivery in association with subsequent childbearing have reported lower rates of subsequent live births among women who delivered by cesarean, even after 10 or more years of follow-up.5,6
Our data suggest that first delivery by cesarean is associated with a lower rate of conception during the first 36 months after first childbirth, which is only partially explained by confounding variables. As the global cesarean delivery rate continues to rise, even a modest level of impairment in women’s ability to conceive and bear children after cesarean delivery has the potential to affect the childbearing patterns of many families, particularly in countries with high cesarean delivery rates. Further large-scale prospective studies are needed that measure unprotected intercourse on a month-by-month basis after first childbirth, to see if our findings can be replicated in different populations and to investigate the extent of specific pathologies following cesarean delivery that could explain lower subsequent conception rates among women who have had a previous cesarean delivery.
Accepted for Publication: February 9, 2020.
Published: April 20, 2020. doi:10.1001/jamanetworkopen.2020.3076
Open Access: This is an open access article distributed under the terms of the CC-BY License. © 2020 Kjerulff KH et al. JAMA Network Open.
Corresponding Author: Kristen H. Kjerulff, PhD, Department of Public Health Sciences, Penn State College of Medicine, 90 Hope Dr, Mail Code A210, Hershey, PA 17033 (email@example.com).
Author Contributions: Dr Kjerulff had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.
Concept and design: Kjerulff, Paul, Weisman, Hillemeier, Repke.
Acquisition, analysis, or interpretation of data: Kjerulff, Paul, Weisman, Hillemeier, Wang, Legro.
Drafting of the manuscript: Kjerulff, Weisman, Hillemeier, Wang, Legro.
Critical revision of the manuscript for important intellectual content: All authors.
Statistical analysis: Kjerulff, Weisman, Wang.
Obtained funding: Kjerulff, Paul.
Administrative, technical, or material support: Kjerulff, Paul, Legro, Repke.
Conflict of Interest Disclosures: Dr Paul reported receiving grants from National Institutes of Health during the conduct of the study. Dr Weisman reported receiving grants from National Institutes of Health during the conduct of the study. Dr Legro reported receiving grants from Guerbet and consulting work from Ferring, Bayer, Fractyl, and Abbvie during the conduct of the study. No other disclosures were reported.
Funding/Support: This study was funded by grant R01-HD052990 from the Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health.
Role of the Funder/Sponsor: The funder had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.
Additional Contributions: We thank the hospitals, outpatient clinics, and other organizations involved with participant recruitment, and the study participants who generously gave their time to participate in the interviews over the course of a 3-year period.
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